Molecular analysis of gene OsCRF1 induced by chilling stress: expression pattern, in vitro ubiquitination assay, and subcellular localization

Chilling (sub-optimal temperature) stress adversely affects plant growth and productivity during rice cultivation period, especially at seedling and reproductive stages. We selected the Oryza Sativa chilling-induced RING E3 ligase gene (OsCRF1) and examined its expression pattern in different tissues during vegetative and reproductive stages.