Ethyl methanesulfonate (EMS)-Induced Mutagenesis of Durum Wheat for TILLING

Mutation breeding is one of the popular and effective tools for crop improvement. There are many published procedures for the mutagenesis of wheat using chemical mutagen such as ethyl methanesulphonate (EMS), but there is no consistency in terms of the optimal conditions for effective EMS mutagenesis. In view of this, the aim of the present study was two-fold: First, to model and optimize the factors influencing the germination and survival rate of EMS-induced durum wheat mutants. Second, to investigate phenotypic variation of M1 and M2 mutant generations.