Efficiency of soaking method on Agrobacterium tumefaciens mediated transformation of LeGSNOR to rice

The Agrobacterium-mediated transformation method has been successfully used to introduce useful foreign genes to plants. Rice is usually transformed using Agrobacterium tumefaciens. Agrobacterium-mediated transformation method mainly introduces and selects callus, this method takes a long time whereby there is a disadvantage that Variants may occur during the cultivation duration.