국내 육성 품종 ‘탐미유채’의 소포자 배양 시 배양조건이 배발생에 미치는 영향

Effects of Culture Condition on Embryogenesis in Microspore Culture of Brassica napus L. Domestic Cultivar ‘Tammiyuchae’

For the establishment of an efficient embryo-genesis from microspore culture in Brassica napus L. domestic cultivar ‘Tammiyuchae’, four different factors affecting microspore embryogenesis and plantlet regeneration were investigated. The highest embryogenesis rate was achieved when microspores at late uninucleate to early binucleate stage were isolated from flower buds with a length of 3.0~3.5 mm. On average, 388 embryos generated from 1 ml of microspores media. The highest number of embryos was obtained when microspores were subjected to 32.5℃ for 2 days. Embryogenesis of ‘Tammiyuchae’ was increased with increasing microspore culture density up to about 5 × 104 ea/mL. Gradually higher culture density repressed embryogenesis of microspores. Regeneration rate of shoots from microspore-derived embryos was observed in MS solid medium supplemented with 0.5 mg ․ L-1 NAA and 1.0 mg ․ L-1 BA, and grew well in MS solid medium without plant growth regulators.