
고-Lysine 보리 돌연변이 계통 M98 종실의 아미노산 조성 및 Proteome Profile 특성
Characterization of Grain Amino Acid Composition and Proteome Profile of a High-lysine Barley Mutant Line M98
- 김대욱(Dea-Wook Kim) 김홍식(Hong-Sik Kim) 박형호(Hyoung-Ho Park) 황종진(Jong-Jin Hwang) 김선림(Sun-Lim Kim) 이재은(Jae-Eun Lee) 정건호(Gun-Ho Jung) 황태영(Tae-Young Hwang) 김정태(Jung-Tae Kim) 김시주(Si-Ju Kim) Randeep Rakwal 권영업(Young-Up Kwon)
- 한국작물학회
- 한국작물학회지
- 제57권 제2호
- 등재여부 : KCI등재
- 2012.06
- 171 - 181 (11 pages)
Lysine is the first limiting essential amino acid in cereals for humans and monogastric animals, although its content is generally low. A chemically induced high-lysine barley mutant, M98, has an agronomically undesirable shrunken en-dosperm trait. In order to obtain detailed insight into the atypical traits of M98 grains, we characterized amino acid composition and protein profiles of M98 and its parent cultivar Chalssalbori. Among a total of 16 amino acids, the percentage of each of the 7 amino acids, including lysine, was 1.2∼1.8 times higher in M98, comparing to Chalssalbori. The percentage of proline and its precursor, glutamic acid, in M98 was about the half of that of the amino acids in Chalssalbori, but arginine synthesized from glutamic acid was 1.8 times higher in M98, compared that in the parent cultivar. Theses results indicated that the mutation in M98 grains might alter the proportion of amino acids linked to each other in a biosynthetic pathway. A comparison of grain proteome profiles between Chalssalbori and M98 revealed 70 differentially expressed protein spots, where 45 protein spots were up-regulated and 25 protein spots down-regulated in M98 compared to those in Chalssalbori. Of these changed protein spots, 53 were iden-tified using nano-electrospray ionization liquid chromatography mass spectrometry. Most of these identified proteins were in-volved in various biological processes. In particular, 28 protein spots such as β-amylase, serpins and B3-hordein were identified as proteins associated with the atypical traits of M98. It was thought that a genetic study on the unique protein profile of M98 would be needed to develop an agronomically feasible barley cultivar with high-lysine trait.
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