Aiming at development of region specialized crop, this study was conducted to clarify variety and culti-vation region dependent on antioxidative compounds in black soybean seeds. For this purpose two black soybean varieties (Ilpumgeomjeongkong and Cheongjakong) were cultivated in 3 different regions (Hwaseong in Gyeonggido, Naju in Jeollanamdo and Jinju in Gyeongsangnamdo) in 2004 and 2005, and harvested seeds were used for isoflavone, antho-cyanin and tocopherol contents along with electron donating ability-based antioxidative activities measurements. 100 grain weight between two varieties were not significantly diffe-rent, but Hwaseong district showed higher 100 grain weight compared to Jinju and Naju. Ilpumgeomjeongkong was higher total isoflavone content (1,064.9 μg/g) compared to Cheong-jakong (801.3 μg/g) in 2004, whereas Cheongjakong showed higher in 2005 compared to Ilpumgeomjeongkong. The highest isoflavone content was obtained in Hwaseong district in 2004, whereas it is the reverse in 2005 that Jinju district showed the highest isoflavone content. In total anthocyanin content, Ilpumgeomjeongkong (7.22 mg/g) was higher than that of Cheongjakong (6.83 mg/g), and Jinju district showed the highest total anthocyanin content (9.16 mg/g) compared to Naju and Hwaseong cultivating districts in their three cultivating districts. Total tocopherol content showed no significant difference between two varieties, but Hwaseong (217.2 μg/g) and Jinju (216.3 μg/g) districts showed higher content compared to Naju (189.7 μg/g) among three culti-vating districts. In tocopherol content ratio, γ-tocopherol was the highest from 56.2% to 59.9%. In electron donating ability (EDA) between two cultivars, Ilpumgeomjeongkong was significantly higher than Cheongjakong, and Naju was the highest of 55.6% among three cultivating districts.
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