검정콩 함유 안토시아닌의 동시분석을 위한 최적 HPLC 분석 조건

Optimal HPLC Condition for Simultaneous Determination of Anthocyanins in Black Soybean Seed Coats

Black soybean has been widely utilized as foods and oriental medicinal materials. The pigmentation in the seed coat of black soybean is due to accumulate antho-cyanins in the epidermis palisade layer. The anthocyanins of black soybean seed coat are considered as a parameter of quality evaluation of black soybean. Therefore, the pur-pose of this study was to investigate the most suitable HPLC condition for simultaneous determination of antho-cyanins in black soybean seed coats extracts. The efficient HPLC analytical condition of D3G, C3G, and Pt3G con-tained extracts of black soybean seed coats was developed. The gradient elution employed a 250 mm × 4.6 mm i.d. YMC-pak ODS-AM 303 column. The gradient system was used two mobile phases. A gradient elution was performed with mobile phase A, consisting of 5% aqueous formic acid, and mobile phase B, comprising 5% formic acid - acetonitrile, and delivered at a flow rate of 0.7 mL/min as follows: 0~35 min, 90% A ~ 60% A; 36 min, 90% A; 46 min, 90% A. The UV-VIS. detection wavelength was set at 520 nm. The limit of detection (LOD) for D3G, C3G, and Pt3G were under 10 ng/mL.