Regulation of Ascorbate Peroxidase Gene Expression in Response to Stresses and Phytohormone in Rehmannia Glutinosa
- Myoung Ryoul Park 박문희(Park Mun Hee) 유창연(Yu, Changyeon) Nam Hee Yoo 윤성중(Yun, Song Joong)
- 제50권 제4호
- 등재여부 : KCI등재
- 286 - 290 (5 pages)
Ascorbate peroxidase (APX) plays a crucial role in the detoxification of hydrogen peroxide. APX activity is maintained significantly higher in the paraquat-treated leaves of the paraquat-tolerant Rehmannia glutinos. This study was conducted to understand structural and regulatory characteristics of APX gene in R. glutinosa. A putative APX cDNA clone (RgAPX1) was isolated from a leaf cDNA library using a partially sequenced expressed sequence tag clone. RgAPX1 is consisted of 1148 bp nucleotides and contains an open reading frame encoding a 250 amino acid-long polypeptide. Deduced RgAPX1 amino acid sequence shares higher sequence similarity to cytosolic APXs. RgAPX1 expression was higher in the leaf than in the flower and root. Southern blot result indicates the presence of one or two RgAPX1-related genes in R. glutinosa genome. RgAPX1 transcription was affected differentially by various stresses and phytohormone. The results indicate that RgAPX1 is constitutively expressed in most tissues and its expression is modulated for the immediate and efficient detoxification of H2O2 under normal and stress conditions.
ascorbate peroxidase (APX);oxidative stresses;paraquat;Rehmannia glutinosa