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KCI등재 학술저널

Efficient Cryopreservation of In Vitro Grown Shoot Tips of Strawberry (Fragaria x ananassa Duch.) Germplasm Using Droplet-Vitrification

DOI : 10.7732/kjpr.2021.34.6.600
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Cryopreservation method using a droplet vitrification was applied to the thirty-one strawberry accessions of in vitro grown shoot tips. A protocol with 0.3 - 0.5 M preculture followed by C4 loading and B1 dehydration solutions efficiently implemented cryopreservation of twenty-six strawberry accessions. The highest regrowth rate was 85.8% for PHS0007 and others were ranged between 85.8% and 21.0%. A slightly modified protocol was applied to five accessions. With these two protocols, twenty-eight accessions obtained more than 40% regrowth rate. This study showed that the droplet vitrification method was able to practically implement cryopreservation of in vitro grown shoot tips of broad range of strawberry germplasm (105).

Introduction

Materials and Methods

Results and Discussion

Acknowledgments

Conflicts of Interest

References

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