
Efficient Cryopreservation of In Vitro Grown Shoot Tips of Strawberry (Fragaria x ananassa Duch.) Germplasm Using Droplet-Vitrification
- 배진주(Jinjoo Bae) 이선이(Sun-Yi Lee) 송재영(Jae-Young Song) 이정로(Jung-Ro Lee) 윤문섭(Munsup Yoon) 이정윤(Jung-Yoon Yi) 김행훈(Haeng-Hoon Kim) 이영이(Young-Yi Lee)
- 한국자원식물학회
- 한국자원식물학회지
- 제34권 제6호
- 등재여부 : KCI등재
- 2021.12
- 600 - 607 (8 pages)
Cryopreservation method using a droplet vitrification was applied to the thirty-one strawberry accessions of in vitro grown shoot tips. A protocol with 0.3 - 0.5 M preculture followed by C4 loading and B1 dehydration solutions efficiently implemented cryopreservation of twenty-six strawberry accessions. The highest regrowth rate was 85.8% for PHS0007 and others were ranged between 85.8% and 21.0%. A slightly modified protocol was applied to five accessions. With these two protocols, twenty-eight accessions obtained more than 40% regrowth rate. This study showed that the droplet vitrification method was able to practically implement cryopreservation of in vitro grown shoot tips of broad range of strawberry germplasm (105).
Introduction
Materials and Methods
Results and Discussion
Acknowledgments
Conflicts of Interest
References