
Improved solid-phase competition enzyme-linked immunosorbent assay for the specific detection of antibodies against porcine parvovirus based on VP2 protein expressed in baculovirus
- 우인옥(In-Ohk Ouh) 이주연(Ju-Yeon Lee) 문서영(Seo Young Moon) 송재영(Jae Young Song) 장상호(Sang Ho Jang) 정광면(Kwang Myun Cheong) 박최규(Choi-Kyu Park) 이윤희(Yoon-Hee Lee)
- 한국예방수의학회
- Journal of Preventive Veterinary Medicine
- Vol.45, No.4
- 등재여부 : KCI등재
- 2021.12
- 158 - 165 (8 pages)
A solid-phase competition enzyme-linked immunosorbent assay (ELISA), recombinant VP2 (rVP2) protein, and monoclonal antibody (mAb) were developed for the specific and sensitive detection of porcine parvovirus (PPV) antibodies in pig sera. A total of 1,544 sera samples were collected from breeding pig farms located in the Gyeongsangbuk-do Province in the Republic of Korea. The optimal operating conditions of SC-ELISA were as follows. The concentration of rVP2 proteins coated on the wells was 4 μg/mL, the swine sera were diluted 1:2, and the HRP-conjugated PPV VP2 mAb (9A8 clone) was used at 500 ng/mL. These results suggest that the SC-rVP-ELISA assay may be a valuable alternative to the current diagnostic tools used to detect PPV-specific monoclonal antibodies and broadly monitor PPV infections in domestic pigs at different breeding stages.
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
ACKNOWLEDGEMENTS
CONFLICT OF INTEREST STATEMENT
REFERENCES