땅콩호박(Cucurbita moschata ‘Butternut’ ) 씨 추출물의 항염증 효능 검증

Anti-inflammatory Efficacy Verification of Cucurbita moschata ‘Butternut’ Seed Extracts

본 연구에서는 버려지고 있는 땅콩호박(Cucurbita moschata ‘Butternut’ )의 씨를 활용하기 위해 70% 에탄올로 추출(CMBE)하여 항산화 및 항염증 검증을 통해 천연 소재로서의 이용가치를 확인하고자 하였다. 땅콩호박 씨의 생리 활성을 확인하기 위해 총 폴리페놀 함량, 전자공여능, 2,2-azino-bis(3-ethyl-benthiazoline-6-sulfonic acid) (ABTS) 라디칼 소거능을 측정하였다. 총 폴리페놀 함량 측정 결과, CMBE는 115.17 ± 0.65 mg/100 g의 총 폴리페놀 함량을 확인하였다. 전자공여능 및 ABTS 라디칼을 측정한 결과, CMBE는 1,000 μg/ml 농도에서 30.01%, 90.52%의 항산화 활성이 나타나는 것을 확인하였다. 대식세포인 RAW 264.7을 이용하여 3-[4,5-dimethyl-thiazol-2-yl]-2,5-diphenyl-tetrazoliumbromide (MTT) assay를 통해 세포생존율을 확인한 결과, CMBE는 100 μg/ml 농도에서 95% 이상의 세포 생존율을 확인하였다. 이후 실험은 세포생존율이 95% 이상인 100 μg/ml 농도 이하에서 진행하였다. 항염증 활성을 검증하기 위해 NO 생성 저해 활성을 측정한 결과, 농도의존적으로 감소함을 확인하였다. 이러한 결과를 토대로, 땅콩호박 씨의 항산화 및 nitric oxide(NO) 생성량 저해 활성을 검증하였으며, 이에 땅콩호박 씨 추출물은 천연 소재로서의 개발 가능성을 가진 것으로 판단되어진다.

In this study, to utilize the seeds of Cucurbita moschata ‘Butternut’, extract it with 70% ethanol (CMBE) and cofirmed the value of use as a natural material through antioxidant and anti-inflammatory verification. To prove the physiological activity of CMBE, total polyphenol content, electron donating ability, and 2,2-azino-bis(3-ethyl-benthiazoline-6-sulfonic acid) (ABTS) radical scavenging ability were measured. As a result of measuring the total polyphenol content, CMBE confirmed a total polyphenol content of 115.17 ± 0.65 mg/100 g. As a result of measuring electron donating ability and ABTS radical scavenging, it was confirmed that CMBE exhibited antioxidant activity of 30.01% and 90.52% at a concentration of 1,000 μg/ml. As a result of confirming cell viability through 3-[4,5-dimethyl-thiazol-2-yl]-2,5-diphenyl-tetrazoliumbromide (MTT) assay using RAW 264.7, CMBE was found to have a cell viability of 95% or more at a concentration of 100 μg/ml. Subsequent experiments were conducted at a concentration of 100 μ g/ml or less. Anti-inflammatory activity was verified through nitric oxide (NO) production inhibitory activity measurement, and it was confirmed that the concentration-dependent decrease was confirmed. Based on these results, the antioxidant and NO production inhibitory activity of Cucurbita moschata ‘Butternut’ seeds was verified, and CMBE was judged to have potential for development as a natural material.