Premna foetida is a woody plant with short and twisted trunk. P. foetida is a scandent, erect shrub or small tree, thorny on the trunk and large branches. Leaves are opposite or whorled and entire or serrate. Premna foetida is a wild plant locally known as “Daun Sebuas”. P. foetida is used for it nutritive and as traditional treatment. The fruit and leaves of P. foetida are prepared for salad. The study aimed at the hydrodistillation and antioxidant activity of leaves, stem-bark and fruits essential oil from Premna foetida Linn, they were analysed by capillary GC and GC-MS. Ninety eight compounds representing 81.68±0.02, 37.31±0.05and 93.45±0.03 of the isolates of leaves, stem-bark and fruits respectively were identified, the most abundant were α –Duprezianene (77.27±0.03, leaves, α-Gurjunene (36.06 ±0.05) fruits and Hinesol acetate (77.19±0.03) stem-bark. Components among which sesquiterpenoids dominated. The total volatiles were assayed for antioxidant potentials using 2, 2-diphenyl-1-picrylhydrazyl (DPPH). The total volatiles showed strong activity with IC50 of 11.74±0.82µg/ml, 9.63±0.34 µg/ml and 49.73±1.12 µg/ml for leaves fruits and stem-bark respectively.
Ⅱ. Materials and method