Evaluation of Genetic Diversity and Comparison of Biochemical Traits of Soybean (Glycine max L.) Germplasm Collections

Assessment of the genetic variation and biochemical traits among various germplasm collections are necessary for utilization of valuable genetic resource and effective strategies of germplasm conservation. The aims of this study were to analyze the genetic diversity using SSR markers and compare protein, oil content and fatty acid composition of about 185 soybean germplasm which is comprised of Korean landraces and collections from six different global regions. Seventy two SSR markers were selected based on their distribution on the 17 genetic linkage groups of soybean. A total of 784 alleles were detected from all accessions, with an average of 10.9 alleles per microsatellite locus. The genetic diversity observed was high wherein three distinct groups were formed containing several subgroups according to their geographic origins. Biochemical contents assay revealed significant differences in their protein and oil contents. Generally, the crude protein content was highest among India and Myanmar collections, while oil content was highest among accessions from USA and China. A negative correlation was observed between protein and oil contents, and between oleic and linolenic acids. The highest value observed for biochemical content among all germplam tested was 45.8% for crude protein, 26.7% for crude oil and 35.7% for oleic acid composition. Our study provided a better understanding of genetic relationships and geographical origin among various germplasm collections, and it could contribute to more efficient utilization of valuable genetic resources.