국내 작물의 배양 가능한 미생물 확인 및 유용 미생물 분리 방법 연구

Investigation of Total Culturable Bacterial Abundance and Isolation of Beneficial Bacteria from Agricultural Crops in South Korea

In this study, we investigated culturable bacterial abundance in various crops. Total culturable aerobic bacteria in tomato plants were enumerated on NA and it showed that the amounts of viable bacterial cells were changed among the different parts of the plants (roots, stems, and fruits), ranging between log 1 and 4 per g sample. To investigate the bacterial abundance dynamics in different crops, culturable aerobic bacterial cell numbers were enumerated on NA and compared between the samples (tomato fruit, sesame leaf, and green onion) harvested freshly from one field trial (Yangsan, Korea). In this trial, the number of total culturable aerobic bacteria of the samples were 8 times higher in sesame leaves and green onions compared to the tomato fruits in the field. Interestingly, culturable aerobic bacteria on MRS were only found in sesame leaves, whereas lactic acid bacteria were not detected in tomato fruit and green onion samples in this field. We also found that the field location (Yangsan, Gangneung, or Busan) influenced the number of culturable aerobic bacteria on the surface of the crops. Tomato samples freshly harvested from the different field locations were tested and showed that the amounts of culturable aerobic bacteria on NA and MRS agar were significantly different based on the field locations of the samples. Finally, lactic acid bacteria were isolated from all the tomato and sesame leaf samples used in this study, showing 20 and 28 morphological diversity. With these isolates, we will be able to conduct further biological and functional investigation to develop a new probiotic strain originated from the crops in Korea.