
A study on the effect of JNJ-10397049 on proliferation and differentiation of neural precursor cells
- Neda Karami Hassan Azari Moosa Rahimi Hadi Aligholi Tahereh Kalantari
- 대한해부학회
- Anatomy and Cell Biology
- Vol.55(2)
- 등재여부 : KCI등재
- 2022.06
- 179 - 189 (11 pages)
The orexin 2 receptor plays a central role in maintaining sleep and wakefulness. Recently, it has been shown that sleep and wakefulness orchestrate the proliferation and differentiation of oligodendrocytes. Here, we explored the role of a selective orexin 2 receptor antagonist (JNJ-10397049) in proliferation and differentiation of neural progenitor cells (NPCs). We evaluated the proliferation potential of NPCs after exposure to different concentrations of JNJ-10397049 by using 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide and neurosphere assays. Moreover, the expression of differentiation markers was assessed by immunocytochemistry and real-time polymerase chain reaction. JNJ-10397049 significantly increased the proliferation of NPCs at lower concentrations. In addition, orexin 2 receptor antagonist facilitated progression of differentiation of NPCs towards oligodendroglial lineage by considerable expression of Olig2 and 2’,3’-cyclicnucleotide 3’-phosphodiesterase as well as decreased expression of nestin marker. The results open a new avenue for future investigations in which the production of more oligodendrocytes from NPCs is needed.
Introduction
Materials and Methods
Results
Discussion
ORCID
Author Contributions
Conflict of Interest
Acknowledgements
References