소나무잔나비버섯의 이너뷰티 소재로서 평가

Evaluated as an Inner Beauty Material for Fomitopsis Pinicola

본 연구에서는 소나무잔나비버섯을 이용하여 이너뷰티 소재로서의 품질과 생리활성을 평가하고자 80℃와 100℃에서 열수 추출하여 색도, pH, 당도를 측정하였으며, DPPH 라디컬소거능, 총 폴리페놀과 총 플라보노이드 함량, ABTS 라디컬 소거능, elastase, α- glucosidase 저해 활성을 측정한 결과 다음과 같다. 소나무잔나비버섯 추출물은 온도가 높을수록 명도가 감소하며, 동일한 추출 시간에서 온도가 높을수록 색도가 증가하였으며, pH 값은 5.31~5.2이며, 당도는 1.0~1,1Brix로 온도에 따라 유의적인 차이는 없었다. DPPH 라디컬 소거능 측정 결과 500 μg/mL의 농도에서 80℃에서 추출한 경우는 60.23%, 100℃에서 추출한 경우는 69.32%의 소거능이 확인되었으며, 총 폴리페놀과 총 플라보노이드 함량은 80℃와 100℃에서 각각 70.71 μg/mL, 89.28μg/mL로 확인되었으며, 총 플라보노이드 함량은 1 각각 34.42μg/mL, 45.07μg/mL로 확인되었다. ABTS 라디컬 소거능은 500 μg/mL의 농도에서 80℃와 100℃에서 각각 65.72, 76.22%의 소거능이 확인되었다. Elastase 저해 효과는 500 μg/mL의 농도에서 80℃와 100℃에서 각각 30.12, 38.63%의 저해 효과가 확인되었으며. α- glucosidase 저해 효과는 58.26%, 62.81%의 저해 효과가 확인되었다. 소나무잔나비버섯 추출물은 온도가 높으면 색과 당도가 증가하고, pH 는 감소하였지만 유의적인 차이는 없었으며, 항산화 효과는 100℃에서 더 높게 확인되었다. 이상의 결과 소나무잔나비버섯은 100℃ 추출온도에서 효과가 더 높게 확인 되어 적절하였지만 추후 연구에서는 관능검사를 통해 색, 향, 탁한 정도, 맛, 전체적인 기호도 등의 평가가 필요하다고 사료된다.

In this study, in order to evaluate the quality and physiological activity of Fomitopsis pinicola as an inner beauty material, hot water extraction was performed at 80°C and 100°C to measure color, pH, and sugar content. DPPH radical scavenging ability, total polyphenol and total flavonoid content, The results of measuring ABTS radical scavenging activity, elastase, and α-glucosidase inhibitory activity are as follows. In the extract of Fomitopsis pinicola, the brightness decreased as the temperature increased. At the same extraction time, the chromaticity increased as the temperature increased. The pH value was 5.31~5.2, and the sugar content was 1.0~1,1Brix. there was no As a result of measuring DPPH radical scavenging activity, at a concentration of 500 μg/mL, the scavenging activity was 60.23% when extracted at 80 °C and 69.32% when extracted at 100 °C. It was confirmed to be 70.71 μg/mL and 89.28 μg/mL, respectively, and the total flavonoid content 1 was confirmed to be 34.42 μg/mL and 45.07 μg/mL, respectively. ABTS radical scavenging activity was confirmed to be 65.72% and 76.22% at a concentration of 500 μg/mL at 80°C and 100°C, respectively. Elastase inhibitory effects were confirmed to be 30.12 and 38.63%, respectively, at 80°C and 100°C at a concentration of 500 μg/mL. The inhibitory effects of α-glucosidase were found to be 58.26% and 62.81%. In the extract of Fomitopsis pinicola, the color and sugar content increased when the temperature was high, and the pH decreased, but there was no significant difference, and the antioxidant effect was confirmed to be higher at 100℃. As a result, the effect of Fomitopsis pinicola was confirmed to be higher at the extraction temperature of 100℃, so it was appropriate.