The purpose of this study was to determine the optimal sowing date for the spring sowing cultivation of rapeseed (Brassica napus L.). To determine suitable rapeseed sowing dates for spring sowing cultivation, changes in growth, flowering characteristics, and seed production were investigated in Muan, South Korea between 2019 and 2020. ‘Jungmo7001’ is the suitable variety for spring sowing because of its early flowering characteristics, high seed yield, and the fact that it occupies the highest cultivation area in South Korea. When the yield of ‘Jungmo7001’ was investigated in 2019 and 2020, the highest yields recorded were 243 kg/10a in February 28, 2019, and 294 kg/10a in February 18, 2020. As a result, the optimal rapeseed seeding period is considered to be mid-to-late February in the southern region. There was no statistically significant difference in seed crude oil content. The content of oleic acid (C18:1) decreased, but that of linoleic acid (C18:2) and of linolenic acid (C18:3) increased significantly depending on the sowing date.