국내 밀 품종의 종자 외관 특성 및 영상 이미지 분석

Analysis of Grain Appearance Traits and Images of Korean Wheat Cultivars

To improve the seed purity management system of Korean wheat cultivars, 50 Korean wheat cultivars were subjected to chemicalassays for grain color, genotyping of grain weight-related genes, and grain image analysis. The tested cultivars were primarily classified byNaOH and ninhydrin tests as white (26%) and red (74%) cultivars, as well as high PPO activity (48%), and low PPO activity (52%) cultivars,respectively. The allelic variations of Tamyb10 gene revealed Tamyb-A1a/Tamyb-B1a/Tamyb-D1a as the major allelic combination in whitewheat and five different Tamyb10 genotypes (i.e., aba, abb, baa, bba, and bbb) in red wheat. Those cultivars with high PPO activity possessedthe Ppo-A1a/Ppo-B1b/Ppo-D1b genotype, while those with low PPO activity possessed the Ppo-A1b/Ppo-B1a/Ppo-D1a genotype. In the grainimage analysis, long grain cultivars displayed increased grain width, circularity, and area. Based on cluster analysis of grain traits, the Koreanwheat cultivars were classified into two groups - 1) large red grain cultivars released before 2000, and 2) small red grain cultivars and whitewheat cultivars released after 2000. Further research is required to determine the effects of grain filling conditions on the grain characteristicsof Korean wheat cultivars and to develop efficient and reliable molecular markers for an improved seed purity management system.