개인식별을 위한 STR 마커의 개발

단일반복 염기서열을 가진 GATA67G11 유전좌의 특성 규명

The development of polymerase chain reaction(PCR) method with short tandem repeat (STR) markers is very useful tool for human identification in forensic cases. PCR method is lots advantages than the Amp-FLP method. This method can be performed by small amount DNA templates, quickly time and low cost. STR marker still concentrates on many forensic scientists since reporting PCR as a human identification method in 1885. In order to optimal condition of PCR amplification with GATA67G11 locus. There is tested condition for various annealing temperature and time, template DNA amount, wide range cycle numbers and primer concentration for optimal amplification. In this study, sensitive PCR was achieved by optimizing parameters such as 0.4 pmole primers, and 10ng template DNA. Optimal amplifying condition of GATA67G11 locus was 61℃ for 20 second. Therefore, it would take a optimal condition for 95℃, 10 sec., annealing for 61℃, 20sec., extension for 72℃, 10sec., repeating 30 cycles before final extension 72℃, 10 min. The sequencing data of small single allele were showed two repetitive units, three TAGG repeating numbers and eight ATAG repeating numbers, 11 repeating numbers totally. In conclusion, optimal PCR condition in GATA67G11 locus was studied to apply the individual human identification with DNA specimens. There resulted in success in all specimens and confirmed many methods for individual identification with GATA67G11 locus.