국내 육성 장미 품종 꽃잎 유래 체세포배 발생 캘러스 유도

The Induction of Somatic Embryogenic Callus from Petals-Derived Callus in Rosa hybrida

This study was conducted to induce somatic embryogenic callus (SEC) derived from petals in rose. The petal explants of 3 cultivars (‘Ice Wing’, ‘Orange Eye’ and ‘Pink Beauty’) with different flower colors were placed on three types media (MS, SH and WPM) supplemented with 11 ㎎/L 2,4-D, respectively, and then cultured in the dark for 47 days. Calluses were formed at explants of all three cultivars. Also, ‘Ice Wing’, which were cultured in the SH as the basal medium, showed the highest callus formation rate. However, somatic embryos were generated from only petal-derived callus of ‘Ice Wing’, which were induced on the WPM as the basal medium, transferred it to SH basal medium supplemented with 3 ㎎/L 2,4-D, and 300 ㎎/L L-proline, and cultured for 5 weeks. The SEC has been proliferated every four weeks at the subculture interval. In addition, as a results of making a comparison of expression of RhSERK3 and RhSERK4, which is used as signal for generation of somatic embryo from callus in rose, between the SEC and petal-derived callus from ‘Ice Wing’ by RT-qPCR, the former showed 10 times higher RhSERK3 expression and 700 times higher RhSERK4 expression than the latter.