대용차 개발을 위한 착색된 배(Pyrus serotina)열매로부터 항산화 화합물의 분리 동정

Isolation and Identification of Antioxidant Compounds from Stained Pyrus serotina Fruit Extract for alternative tea development

착색 발효된 배 건조물 2 kg을 70 % MeOH 수용액으로 추출하고, 얻어진 추출물을 EtOAc n-BuOH 및 물로 용매 분획 하였다. 이 중 활성이 확인된 EtOAc 분획으로부터 silica gel과 octadecyl silica gel(ODS) column chromatography로 정제하여 2종의 화합물을 분리 하였다. 화합물의 화학구조는 NMR, MS 및 IR 등의 스텍트럼 데이터를 해석 하여 quercetin (1), geniposide (2) 으로 동정 하였다. 항산화 효능 평가에서는 DPPH 억제 효능시험과 ROS 생성 억제 효능 시험을 수행하였다. 먼저, DPPH 억제 효능시험에서 2종의 시료를 최고농도 50 ppm까지 DPPH 활성을 측정한 결과 NICS-1과 NICS-2의 IC50값은 각각 25.554 ppm, 22.943 ppm으로 측정되었다. ROS 생성 억제 효능은 2종의 시료 모두 농도 의존적으로 ROS 생성을 감소시켰으며, UV 조사군 대비 통계적으로 유의하게 ROS 생성을 억제시켰다.

For alternative tea development, 2 kg of dried stained Pyrus serotina was extracted with 70 % MeOH aqueous solution, and the extract was solvent-fractionated with EtOAc, n-BuOH and Water. Two types of bioactive compounds were separated from the EtOAc fraction, by purification with silica gel and octadecyle silica gel (ODS) column chromatography. The chemical structure of the compounds were identified as quercetin (1) and geniposide (2) by analyzing spectral data with NMR, MS and IR. For the evaluation of antioxidant activity, DPPH inhibitory efficacy test and ROS production inhibitory efficacy test were performed. First, as a result of measuring the DPPH inhibitory efficacy of two samples up to the highest concentration of 50 ppm in the DPPH inhibition efficacy test, the IC50 values of NICS-1 and NICS-2 were measured to be 25.554 ppm and 22.943 ppm, respectively. In ROS production inhibitory efficacy test, the ROS production was reduced in a concentration-dependent manner in both samples, and statistically significantly inhibited ROS production compared to the UV irradiation group. According to recent reports, this study is expected to develop eco-friendly sustainable health drinks using pears and cheese and functional raw materials using pears and teeth through organic cosmetics.