포도 ‘Tano Red’ בRuby Seedless’ 집단에서 SNP 분자표지를 이용한 유전자지도 작성

Construction of Genetic Linkage Map in Grape ‘Tano Red’ (Vitis labrusca × V. vinifera) × ‘Ruby Seedless’ (V. vinifera) F1 Population Using SNP Markers

Grape (Vitis vinifera L.) is a perennial fruit tree with high heterozygosity, consisting of 38 chromosomes (2n=38), and it takes a long time for grape seedlings to grow into fruit-bearing trees. Therefore, it is difficult to study grape genetics and breeding strategies. However, it has recently become possible to discover many SNPs through whole genome resequencing or genotyping-by-sequencing (GBS) analysis. In this study, we aimed to develop high-resolution melting (HRM) markers from the detected SNPs and construct a genetic linkage map using HRM markers. In a previous study, 2,553 SNPs were identified using GBS analysis. In this study, 1,336 SNPs were used to design primer sets for HRM analysis. The developed HRM markers were used for construction of a genetic linkage map in an F1 segregating population consisting of 192 individuals from a cross between ‘Tano Red’ (V. labrusca × V. vinifera) and ‘Ruby Seedless’ (V. vinifera). A total of 805 polymorphic HRM markers were developed, of which 363 were mapped onto the genetic linkage map of grape, with a total length of 1,453.5 cM consisting of 19 chromosomes. This SNP-based genetic linkage map and HRM markers can be used for QTL identification and marker development for important fruit traits of grape.