GBS-SNP와 SSR을 이용한 배 ‘만풍배’와 ‘대원홍’ 종간 교배 집단의 고밀도 유전자 연관 지도 작성

Construction of High-Density Genetic Linkage Map Using GBS-SNPs and SSRs in Interspecific Hybrid Derived from the Cross Between ‘Manpungbae’ and ‘Oharabeni’ Pear (Pyrus spp.)

Pear (Pyrus spp.) is an economically important fruit tree that grows extensively worldwide. To facilitate the identification of agronomically important traits and provide new information for genetic and genomic research concerning this fruit tree, a high-density genetic linkage map of pear was constructed using 178 F1 populations derived from a cross between ‘Manpungbae’ and ‘Oharabeni’. Single nucleotide polymorphisms (SNPs) detected by genotyping-by-sequencing (GBS) and simple sequence repeats (SSRs) developed from pears were analyzed to construct a genetic linkage map. SSR markers were used to locate the corresponding chromosome number for each linkage group (LG). A total of 1,807 GBS-SNPs and 41 SSRs were anchored to the integrated genetic linkage map. Seventeen LGs were identified, covering a genetic distance of 1,519.4 cM with an average marker density of 0.87 cM. The lengths of the LGs ranged from 70.9 cM (LG 14) to 160.4 cM (LG 15). Each LG had SSR markers f rom 1 to 5 , except f or LGs 7 , 8, a nd 9 . Our integrated g enetic map o f pear c ould b e used a s a b asic f rame map for comparative analysis of genomic structure between different pear research groups.