Dormancy-breaking and Germination of Chelidonium majus L. subsp. asiaticum H. Hara Seeds by Stratification and Gibberellins

Dormancy-breaking and Germination of Chelidonium majus L. subsp. asiaticum H. Hara Seeds by Stratification and Gibberellins

The demand for Chelidonium majus L. subsp. asiaticum H. Hara is expected to increase due to its pharmacological properties such as antibacterial, antioxidant, and anti-inflammatory effects. However, an effective propagation system for this species has not yet been established. This study was conducted to analyze the seed dormancy and germination characteristics of C. majus L. subsp. asiaticum H. Hara native to Korea and establish a mass propagation system. The dormancy type was primarily classified by analyzing the general information of the collected seeds. The seed dormancy breaking was investigated by comparing the effects of cold stratification (0, 2, 4, 8, 10, or 12 weeks) with warm stratification (S, summer temperature, 25/15°C) and intermediate temperature stratification (A, autumn temperature, 15/10°C) of alternating temperature stratification (S12-A4 or S12-A8 weeks). After dormancy break, 500 ㎎/L GA3 and GA4+7 treatment replaced cold stratification and improved seed germination. The results of this study are expected to provide basic data for future seed propagation and mass propagation by analyzing the dormancy and germination characteristics of C. majus L. subsp. asiaticum H. Hara seeds.