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학술대회자료

청국장에서 분리한 바실러스종으로부터 베타-글루코시다제의 분석 및 분리정제

※해당 콘텐츠는 기관과의 협약에 따라 현재 이용하실 수 없습니다.

The strain obtained from Cheonggukjang produced a protein showing β-glucosidase activity in the experimental data of column chromatography and sodium dodecyl sulphate-polyacrylamide gel electrophoresis. An extracellular β-glucosidase of Bacillus species isolated from Cheonggukjang was identified and partially purified through 75% ammonium sulfate precipitation, High Q anion exchange chromatography, and Bio-Gel P-30 and P-60 size exclusion chromatography. The molecular weight of 45,000 was estimated by SDS-PAGE. β-Glucosidase was produced in the culture medium containing cellobiose, but not glucose. implying an inhibitor. Assay of β-glucosidase activity was performed by measuring the release of p-nitrophenol from the substrate p-nitrophenyl-β-D-glucopyranoside.

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