OBJECTIVES The purpose of present study was to analyze the acute effects of exergame on glucose control after glucose ingestion in individuals with pre- and type 2 diabetes. METHODS We enrolled 60 adults with pre-diabetes (Pre-DM, 58.1±9.2 years, n=20) and type 2 diabetes (T2DM, 61.7±9.9 years, n=40). We measured fingertip capillary blood glucose level at 10 min before, 30 min after, 60 min after and 120 min after the 75-g oral glucose tolerance test (OGTT). For the non-exergame control days, participants pursued normal daily activities but refrained from unusual strenuous physical activity. On the exercise days, participants conducted on exergame at 30 min after 75-g OGTT for 30 min. RESULTS Acute exergame reduced the glucose level 60 min, ΔBG30-60 and ΔBG60-120 after glucose ingestion compared to the control trials (p<0.01, p<0.01 and p<0.001 respectively). The cumulative glucose total area under the curve was lower with exergame than under the control condition (p<0.05). CONCLUSION Acute exergame after glucose ingestion can improve hyperglycemia and glycemic excursion.