In Vitro Anticancer Effect of Gardenia jasminoides Ellis Extract on HepG2 Human Liver Cancer Cells

In Vitro Anticancer Effect of Gardenia jasminoides Ellis Extract on HepG2 Human Liver Cancer Cells

摘 要：本研究旨在评估栀子提取物对癌细胞的生理活性作用，并采用 MTT 法和 qPCR 法检测栀子对HepG2 肝癌细胞体外增殖抑制和凋亡诱导效应。结果表明，栀子可抑制 HepG2 细胞增殖，而对 L02 正常肝细胞无毒性，不影响其增殖。这一发现揭示了栀子提取物作为潜在的抗癌药物的价值。同时，栀子具有上调 HepG2 细胞 caspase-3、caspase-8 以及 caspase-9 的表达的作用，这进而促进了癌细胞的凋亡。栀子是富含生物活性成分的一种天然药物，并且它还具有良好的抗癌效果。

This study's objectives were to assess the physiological effects of Gardenia jasminoides Ellis extract on cancer cells and to use MTT and qPCR to identify the extract's in vitro induction of apoptosis and suppression of cell proliferation in HepG2 hepatoma cells. The findings shown that while Gardenia jasminoides Ellis is non-toxic to L02 normal liver cells and has no effect on their proliferation, it can suppress the growth of HepG2 cells. The importance of Gardenia jasminoides Ellis extract as a possible anticancer medication is made clear by this result. In the meantime, Gardenia jasminoides Ellis increases the production of caspase-3, caspase-8, and caspase-9 in HepG2 cells, which in turn encourages cancer cells to undergo apoptosis. A natural remedy with several bioactive components, gardenia jasminoides also has potent anti-cancer properties.