케냐 니에리 그린빈(Green Coffea Arabica beans in Kenya Nyeri)의 피부노화에 관한 기초자료 연구

A Study on Basic Data on Skin Aging of Green Coffea Arabica Beans (in Kenya Nyeri)

케냐 니에리산 커피 생두(Green Coffea Arabica Beans) 열수 및 70% 에탄올 추출물의 방사선조사 전, 후의 생리활성을 검증하여 천연소재로서의 활용 가능성을 확인하였다. 총 폴리페놀 함량측정 결과, 방사선 조사 전의 케냐 니에리산 커피생두 열수 추출물(GBKW)은 297.05 ± 0.99 mg/100 g, 70% 에탄올 추출물(GBKE)은 671.34 ± 0.75 mg/100 g 함량을 나타내었고, 방사선조사(25 kGy) 후의 케냐 니에리산 커피생두 열수 추출물(GBKW-r)은 423.47 ± 0.42 mg/100 g, 70% 에탄올 추출물(GBKE-r)은 415.33 ± 0.79 mg/100 g의 함량을 나타내었다. 전자공여능 측정결과, 500 μg/ml 농도에서 GBKW 및 GBKE는 각각 85.73%, 89.39%, GBKW-r 및 GBKE-r는48.31%, 68.42%의 항산화능을 보여주었다. 또한 ABTS radical 소거능 측정 결과, 500 μg/ml 농도에서 GBKW, GBKE는 52.66%, 67%, GBKW-r, GBKE-r는 99.17%, 99.96%의 소거능을 확인하여 방사선 조사 전, 후 추출물 모두 항산화 활성이 있는 것으로 확인되었다. 이후 대식세포인RAW 264.7을 사용하여 MTT assay를 통해 세포 생존율을 측정한 결과, GBKW-r 및 GBKE-r는10 μg/ml 농도에서 95% 이상의 세포 생존율을 보여주었으며, 이후 세포 실험은 95% 이상의 세포생존율을 보인 10 μg/ml 이하의 농도에서 진행하였다. GBKW-r 및 GBKE-r의 NO 저해 활성은10 μg/ml 농도에서 각각 10.99%, 35.66%를 나타내었으며, GBKW-r 및 GBKE-r는 항염증 효능이 있음을 확인하였다. 이로 인해 방사선 조사 후 케냐 니에리산 커피 생두 열수 및 70% 에탄올 추출물은 항산화 및 항염증 활성이 있음을 확인하여 천연 소재로서의 응용 가능성을 있다고 사료된다.

Physiological activity of Green Coffea Arabica beans (Nieri, Kenya) hot water and 70% ethanol extract before (GBKW, GBKE) and after 25 kGy (GBKW-r, GBKE-r) irradiation was verified to confirm the possibility as a natural material. As a results of measuring the total polyphenol content, GBKW and GBKE extract confirmed the content of 297.05 ± 0.99 mg/100 g and 671.34 ± 0.75 mg/100 g. And GBKW-r and GBKE-r extract confirmed the content of 423.47 ± 0.42 mg/100 g and 415.33 ± 0.79 mg/100 g. As a result of measuring the electron donating activity, it was confirmed that at a concentration of 500 μg/ml, GBKW and GBKE were 85.73% and 89.39%, GBKW-r and GBKE-r showed 48.31% and 68.42%. In addition, the results of ABTS radical scavenging activity, it was confirmed that at a concentration of 500 μg/ml, GBKW and GBKE were 52.66% and 67%, GBKW-r and GBKE-r showed 99.17% and 99.96%, respectively. So, all extracts GBKW, GBKE, GBKW-r and GBKE-r were found to have antioxidant activity. Subsequently, as a result of measuring cell viability through MTT assay using RAW 264.7, which is a macrophage, GBKW-r and GBKE-r showed a cell viability more than 95% at a concentration of 10 μg/ml. Afterwards, NO production inhibitory activity was conducted at a concentration of 10 μg/ml or less. As a result, it was confirmed that GBKW-r and GBKE-r showed anti-inflammatory effects of 10.99% and 35.66%, respectively, at a concentration of 10 μg/ml. Therefore, irradiatied Green Coffea Arabica Beans (in Kenya Nyeri) hot water and 70% ethanol extract have antioxidant and anti-inflammatory activity, and it is considered that there is a possibility of application as a natural material.