Genetic Purity Analysis Using Polymorphic SSR Markers in Rice Genotypes (Oryza sativa L.) and Their Confirmation for the Parental Lines

Genetic Purity Analysis Using Polymorphic SSR Markers in Rice Genotypes (Oryza sativa L.) and Their Confirmation for the Parental Lines

The purity of seeds can be identified from the traits inherited from their parental lines. Hence, contamination may occurat the crossing step due to unshared similarities with their parents. This research aims to measure the genetic purity of several genotypesobtained from crosses between upland and lowland rice through the banding pattern differences among the genotype samples by usingSimple Sequence Repeats (SSR) markers. Taking the leaf samples was carried out at the experimental field, while the marker analysiswas conducted in the Plant Biotechnology Laboratory. In this research, 8 (eight) genotypes obtained from crossing, comprising F1, F2,and BC1 along with 4 (four) of their parents from upland rice and lowland rice, were tested using 6 (six) drought-specific SSR primersof RM5, RM211, RM232, RM249, RM255, and RM258. The banding pattern of the electrophoresis results on the 12 rice genotypesshowed clear, unsmeared quality. Based on the results of distance and genetic similarities, the 12 genotypes could be classified into 4(four) clusters in the dendrogram. Cluster I consists of the Situ Bagendit and BC1 TWCH varieties, Cluster II the Towuti variety, ClusterIII the Ciherang, Cibogo, F1 SBCH, F1 SBCB, and F1 TWCH varieties, and Cluster IV the F2 SBCH, F2 TWCH, F2 SBCB, and BC1SBCH varieties.