더덕 및 씀바귀 참조전사체 단일 파일 DB 구축

Construction of a Single File Reference Transcriptome Database for Deodeok (Codonopsis lanceolata) and Sseumbagwi (Ixeridium dentata)

In this study, we constructed a reference transcriptome database for the special crops Codonopsis lanceolata (known as “deodeok”in Korean) and Ixeridium dentata (known as “sseumbagwi” in Korean). The reference transcriptome sequence based on PacBio Iso-Seq technologywas rapidly analyzed using various programs on a supercomputer at the Rural Development Administration to determine homologous genesearches, domain searches, and metabolic pathway-related information. Transcriptome information was organized into a single file database(DB) with a simple user interface using Microsoft Access, enabling efficient access and management of transcriptome sequences and functionalprediction information. The deodeok reference transcriptome DB included 49,677 transcriptome sequences, whereas the sseumbagwi referencetranscriptome DB included 73,757 transcriptome sequences. These reference transcriptome DBs can be utilized for various genomic analyses,such as gene discovery, gene expression analysis, inter-and intraspecies comparisons, and marker development, with particular potential foruse in secondary metabolite research. The reference transcriptome DBs constructed in this study are expected to contribute to enhancing theagricultural value and industrial applicability of deodeok and sseumbagwi