Cultivar Identification of 55 Korean Peanut Varieties via Selection of Informative SNP Markers from Microarray Genotyping

Cultivar Identification of 55 Korean Peanut Varieties via Selection of Informative SNP Markers from Microarray Genotyping

Peanut variety identification is essential for protecting the intellectual property rights of researchers, ensuring qualitymanagement for producers, and safeguarding the interests of seed production stakeholders. In this research, we developed a molecularmarker set for peanut variety identification using single nucleotide polymorphism (SNP) markers. We used genotyping data andselection procedures, including decision tree and optimal combination selection, to identify a minimal set of informative SNP sites. These SNPs were then converted into Kompetitive allele-specific PCR (KASP) markers. We selected a subset of 14 informative SNPsfrom a pool of 22 candidate markers, representing the minimum number of combinations required to distinguish cultivars. SNPsobtained from the microarrays were converted to KASP markers and then evaluated across 51 peanut varieties. The developed markerset, which consists of a minimal number of markers, is expected to be a rapid and cost-effective tool for peanut variety identification.