α-Amylase와 Protease 효소 활성을 가진 Bacillus subtilis 균주를 이용한 기능성 소재 개발

Development of Functional Material by Using Bacillus subtilis Harboring α-Amylase and Protease Enzyme Activity

Bacillus subtilis MGE 2012 strain was selected for the production of both digestive enzymes, α-amylase and protease, on nutrient agar plates supplemented with starch or skim milk as substrates, and the optimal composition of materials was investigated using combinations of brown rice powder, soybean powder, and rice protein powder. The final fermentation was tested for its antioxidation activity and bifidogenic growth stimulation activity. B. subtilis MGE 2012 simultaneously produced both digestive enzymes, and a combination of brown rice powder and soybean powder was confirmed to be the optimal material for the highest production of the enzymes. The fermentation under the optimized condition showed increased antioxidation activity and bifidogenic growth stimulation activity for Bifidobacterium lactis and Bifidobacterium breve strains when compared with controls. These results indicate the fermentation presented multi-functions including digestion enzyme activity, anti-oxidation activity, and bifidogenic growth stimulation activity and could be used as a healthy ingredient for the human gut.