엘더베리 복합추출물이 함유된 프로바이오틱스 효능 평가

Evaluation of Probiotics Efficacy Based on Elderberry Complex Extract in Vitro for Improving Immunity of Companion Animals

Since COVID-19 pandemic, interest in lactic acid bacteria is increasing as a major health functional food as people recognize the importance of enhancing immunity in terms of physical and mental health of humans as well as pets. We aim to evaluate the efficacy of probiotics that can help improve immunity formulated using elderberries, chicory, bananas, and grains in vitro. Elderberries, chicory roots, and banana complex extracts were fermented using lactic acid bacteria and grains were added to use as samples for this study. Elderberry, chicory root, and banana complex extracts were fermented using lactic acid bacteria and grains were added to use as samples in this study. The antioxidant activity, cell viability, NO production, and inflammatory cytokine production were measured by ABTS radical scavenging activity, MTT assay, and nitrite (NO2-) measurement using LPS-stimulated raw264.7 cells. For evaluating the antioxidant efficacy using ABTS scavenging ability, it was confirmed that the radical scavenging ability was excellent performance depending on the concentration of the sample and the MTT assay performance result also did not affect the cell viability. The NO production inhibitory effect was confirmed depending on the concentration of cells induced by LPS. In addition, the expression amounts of TNF-α and IL-1β decreased as the indicators of cell anti-inflammatory efficacy. The antioxidant and anti-inflammatory activities of the sample used in this study can be identified, and it seems that it can be used as a material with the function of an immune-enhancing aid against diseases such as reduced immunity.