Background: Vibrio vulnificus is a serious opportunistic human pathogen that has a worldwide distribution in a variety of marine and estuarine environments. Objectives: For this reason, we investigated the distribution of Vibrio vulnificus in coastal areas of Gyeonggido Province from 2018 to 2022. Also, we analyzed the correlation between V. vulnificus leading to infection and two marine environmental factors (water temperature and salinity). Methods: We collected a total of 266 samples from six coastal area points (i.e., seawater, mudflats). Specimens were isolated using selective plating media and isolated strains were identified by a VITEK 2 system. To find the relevance of the isolation rates of V. vulnificus and number of cases of V. vulnificus infection, we summarized the data on 48 cases of V. vulnificus infection from the open data of the Korea Disease Control and Prevention Agency. Results: Among the 266 samples taken during the investigation period, 47 strains were isolated, and the separation rates of V. vulnificus were 17.7%. The monthly isolation rates of V. vulnificus were ranked in the order of August (53.8%), September (33.3%), June (28.6%), and July (21.1%). There was a positive correlation with the temperature of seawater, but salinity was not significant. The number of cases of V. vulnificus infection reported in Gyeonggi-do Province were 18 (37.5%) in September, 14 (29.2%) in August, and eight (16.7%) in October. The proportion was 83.3%. It was relevant to the isolation rates of V. vulnificus in the marine environmental sources. Conclusions: Our data showed that the number of V. vulnificus infection cases could be affected by changes in the distribution of V. vulnificus due to rise the temperature of seawater in the marine environment.