Improvement of Lyophilized Probiotics Viability Using a Histidine-based Rehydration Formula
Improvement of Lyophilized Probiotics Viability Using a Histidine-based Rehydration Formula
- 한국유산균프로바이오틱스학회
- Current Topics in Lactic Acid Bacteria and Probiotics
- 10권 1호
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2024.0616 - 26 (11 pages)
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DOI : 10.35732/ctlabp.2024.10.1.16
- 9
Bacteria get stressed and damaged during freeze-drying process for commercialization and this result in loss of its effect. Viability is important for its efficacy, but this drying process can deteriorate viability by damaging the integrity of the cell membrane as well. In this study, we propose 0.03 M histidine for rehydration of freeze-dried probiotics to improve their viability. The freeze-dried bacteria mixture with 0.03 M histidine showed augmented survivability during in vitro simulated gastric and duodenum stress conditions and increased viability during 60 min rehydration. It exhibited a significantly increased adherence ability of lyophilized bacteria to the HT-29 cell-line. Therefore, this shows possibility of probiotics commercialization with damage of lyophilization restored and survivability ameliorated.
Introduction
Materials and Methods
Results
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