Coenzyme A transferase Fn1856-Fn1857 from Fusobacterium nucleatum: purification, crystallization, and X-ray crystallographic analysis

Coenzyme A transferase Fn1856-Fn1857 from Fusobacterium nucleatum: purification, crystallization, and X-ray crystallographic analysis

Fusobacterium nucleatum (F. nucleatum) is one of the most abundant gram-negative anaerobic bacteria, which has long been found to cause opportunistic infections and has recently been implicated in colorectal cancer (CRC). In F. nucleatum, both the CoA transferases (Fn1856, Fn1857) play a crucial role in the butyrate metabolism pathway. Through investigation, the formation of complexes involves the execution of the functions of Fn1856 and Fn1857. In this study, the Fn1856-Fn1857 complex was successfully co-expressed and purified using Ni-NTA affinity and gel-filtration chromatography and then crystallized. The crystal diffracted to 2.80 Å resolution and belonged to the space group P65, with unit cell parameters of a = 166.518 Å, b = 166.518 Å, and c = 191.377 Å, α = β = 90.00° and γ = 120°. The asymmetric unit contained approximately eight monomers of Fn1856-Fn1857.