국화에서 전신발현 프로모터의 구명

The Characterization of Constitutive Promoters in Chrysanthemum (Chrysanthemum morifolium Ramat)

Chrysanthemum is the most popular ornamental plant, after roses and lilies. Thecauliflower mosaic virus (CaMV) promoter, which remains the most widely used promoterin dicotyledons, is a very strong promoter with sufficient effects in most crops. However,weak expression has often been reported in Chrysanthemum. Therefore, we searched forconstitutive promoters available in Chrysanthemum. Based on the transcriptome analysisdata of Chrysanthemum, nine constitutively expressed genes were selected, and eachpromoter region (1.0-3.0 kb) was isolated by genome walking. Only two of the nine promotersexpressed GUS in tobacco and chrysanthemums. The major motif of the CmERFpromoter (U41, 2060 bp) was related to the regulation of ethylene (ERELEE4) or gibberellin(PYRIMIDINEBOXOSRAMY1 and WRKY71OS). Similarly, the motif of the CmGA2 ox promoter(U47, 1060 bp) also contained gibberellin signaling factors, such as PRIMIDINEBOXHVEPB1and WRKY71OS. Both promoters showed strong systemic expression in tobacco usingGUS staining. Although weaker than in tobacco, significant expression was confirmed inthe flowers and stems in chrysanthemum. The results of the GUS activity assay usingchrysanthemum transformants showed that the transgenic line (#12) containing the U47promoter had higher expression in all tissues than that containing the 35S-CaMV promoter. The U41 promoter was found to have a higher expression than the 35S-CaMV promoter inthe stem.