PCR 마커를 이용한 국내 밀 품종의 고분자 글루테닌 Glu-B1 대립유전자 조성 평가

Assessment of Allele Composition of HMW-GS Glu-B1 Locus in Domestic Wheat Cultivars Using PCR-based Markers

In common wheat (Triticum aestivum L.), the protein content and glutenin proteincomposition are the key quality-determining parameters. Allelic variations, especially inhigh-molecular-weight glutenin subunits (HMW-GSs), affect bread quality significantly. The HMW-GS Glu-1 locus consists of two tightly linked genes encoding x- and y-typesubunits that exhibit highly variable frequencies. In this study, we evaluated Glu-B1 allelesusing allele-specific PCR markers in 44 domestic wheat cultivars. The composition ofGlu-1Bx7+Glu-1By8 in the 24 cultivars was either Glu-1Bx7+Glu-1By8, Glu-1Bx7*+Glu-1By8, or Glu-1Bx7*+Glu-1Bx8*. In addition, the two cultivars initially identified Glu-1Bx7+Glu-1By8* were corrected to Glu-1Bx7*+Glu-1By8*. Seven cultivars previouslyclassified as having Glu-1Bx7+Glu-1By9 composition contained Glu-1Bx7*+ Glu-1Bx9. The allele composition of the cultivar was identified as Glu-1Bx20+Glu-1By20 instead ofGlu-1By20. The HMW-GSs of 21 wheat varieties were analyzed using ultra-performanceliquid chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. These results will be helpful for evaluating the composition of Glu-B1 alleles in domesticwheat and accurately assessing the quality of domestic wheat flour.