반응표면분석법을 이용한 미선나무 잎의 verbascoside 및 rutin 추출 조건 최적화

Optimization of Extraction Conditions for Verbascoside and Rutin from Abeliophyllum distichum Nakai. Leaf Using Response Surface Methodology

본 연구는 자원식물로 개발되고 있는 미선나무의 지표성분인 verbascoside와 rutin의 효율적인 확보를 위해 반응표면분석을 이용하여 최적 추출 조건을 확인하고자 수행되었다. 열풍건조된 미선나무 잎을 Box-Behnken design (BBD)에 따라 3 가지 독립변수(에탄올 농도, 추출 시간, 추출 온도)를 설정하여17개 구간에 대해 실험을 수행하였으며, 종속변수로는 verbascoside와rutin의 함량을 측정하였다. Verbascoside의 최적 추출 조건은 에탄올 농도 60%, 추출 시간 14.4 h, 추출 온도 75℃ 였다. 최적 추출 조건에서 예상된 verbascoside의 값은 193.92 ㎎/g이었으며, 실험값은 193.66±4.37 ㎎/g이었다. Rutin의최적 추출 조건은 에탄올 농도 52%, 추출 시간 30 h, 추출 온도75℃였다. 최적 추출 조건에서 예상된 rutin의 함량은 13.67 ㎎ /g이었으며, 실제 실험을 통해 얻은 값은 14.38±0.31 ㎎/g이었다. 반응표면분석법으로 도출된 최적 추출 조건을 통해 verbascoside와rutin의 효율적인 확보가 가능함을 확인하였다.

This study was conducted using response surface analysis to confirm the optimal extraction conditions for efficient supply of verbascoside and rutin, which are indicator components of Abeliophllum distichum Nakai., which is being developed as a resource plant. An experiment was performed on 17 sections using hot air dried leaves of A. distichum by setting three independent variables (ethanol concentration, extraction time, extraction temperature) and the contents of verbascoside and rutin were measured. The optimal extraction conditions for verbascoside were 60% ethanol concentration, 14.4 h extraction time, and 75°C extraction temperature. The expected value of verbascoside under optimal extraction conditions was 193.92 ㎎/g, and the experimental value was 193.66±4.37 ㎎/g. The optimal extraction conditions for rutin were 52% ethanol concentration, 30 h extraction time, and 75°C extraction temperature. The expected rutin content under optimal extraction conditions was 13.67 ㎎/g, and the value obtained through the actual experiment was 14.38±0.31 ㎎/g. It was confirmed that verbascoside and rutin could be efficiently secured through optimal extraction conditions derived by response surface analysis.