두개천골산삼 약침의 항산화 및 Nitric Oxide 소거능에 대한 연구

Study on the Anti-Oxidant and Nitric Oxide Scavenging Effect of Craniosacral Wild Ginseng Pharmacopuncture

Objectives Craniosacral therapy (CST) is more effective when used in combination with pharmacopuncture. Therefore, we tried to develop pharmacopuncture suitable for CST. Methods Antioxidant activity was compared by 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino bis (3-ethylbenzothiazoline- 6-sulfonic acid) (ABTS), and ferric reducing antioxidant power (FRAP) assays for craniosacral wild ginseng pharmacopuncture (CSW), craniosacral pharmacopuncture (CS), wild ginseng pharmacopuncture (WG), distilled craniosacral wild ginseng pharmacopuncture (CSWD), and distilled wild ginseng pharmacopuncture (WGD). And the nitric oxide (NO) scavenging activity of CSW and WG was evaluated to determine the effect of CSW and WG on NO modulation. The effective compounds contained in CSW and WG were quantitatively analyzed by high performance liquid chromatography (HPLC). Results In antioxidant activity, CSW and CS had strong antioxidant activity, while distilled extract pharmacopunctures had very low activity. When wild ginseng and other herbal medicines were used together, the activity was higher than when wild ginseng alone was used. The results of NO scavenging activity was concentration-dependent, and CSW was more active than WG at all concentrations. Nodakenin and paeoniflorin were detected only in CSW. Ginsenoside Rg1 and Rb1 were higher in CSW than in WG. Conclusions CSW contained many kinds and high content of effective compounds due to proper prescription and extraction method. Therefore, various activities were higher. CSW will be more effective when used in conjunction with CST. Additional detailed studies such as mechanism and standardization are needed.