Allelic Variation in HMW-GS of Mongolian Wheat Varieties Using KASP Molecular Marker

Allelic Variation in HMW-GS of Mongolian Wheat Varieties Using KASP Molecular Marker

The storage proteins in wheat, particularly the high molecular weight glutenin subunits (HMW-GS), play crucial roles in the processing of flour and the quality of bread made from common wheat. These subunits are encoded by the Glu-A1, Glu-B1, and Glu-D1 loci. This study aimed to determine the composition of high molecular weight glutenin subunits in 89 Mongolian cultivars of spring wheat using KASP markers and sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The result showed the presence of the 5+10 allele at the Glu-D1 locus in Mongolian-developed varieties was confirmed through SDS-PAGE, providing evidence for the synthesis of high molecular weight glutenin subunits. The uniform presence of high molecular weight glutenin subunit alleles across the tested cultivars contributes valuable insights into the bread-making quality of Mongolian wheat varieties.