신속 요소 분해 효소 검사의 시험액 내 미네랄 이온 농도 변화가 발색반응 속도에 미치는 영향: Helicobacter pylori 감염의 연관성

Sodium, Potassium, Calcium and Magnesium Affect Colorimetric Changes of Rapid Urease Test: Implication on Helicobacter pylori Infection

Helicobacter pylori is a Gram-negative bacterium that is found in the stomach, infection of which is strongly correlated with the occurrence of chronic gastritis, gastric ulcers, and gastric cancer. To endure in acidic environment of mucus layer of stomach, H. pylori uses several ways and secretion of urease to neutralize gastric acid by producing ammonia from urea is among the key features. With this, rapid urease test was widely used to detect H. pylori infection using biopsy specimens. In this study, we explored several supplements that can facilitate a colorimetric change of rapid urease test to shorten the necessary time to confirm a test result. As a result, passive lysis of bacterial barrier using lysozyme, NP-40, or SDS did not facilitate the reaction and even showed inhibitory effect probably due to the inactivation of urease. Addition of low concentration of adenosine tri-phosphate slightly enhances the reaction. Surprisingly, supplementation of sodium and potassium ions prompts the completion of the reaction. In contrast, supplementation of calcium and magnesium ions suppresses the reaction. These results suggest that above ions affect necessary diagnosis time for rapid urease test in detection of H. pylori and implicate the mechanistic insight how dietary supplementation of inorganic ions such as high-salt diet promotes the infection of H. pylori.