Spermidine 처리 조건에 따른 돼지 체외수정란의 초기배 발달 및 배반포 형성에 미치는 영향

Effects of Spermidine Treatment on Early Embryo Development and Blastocyst Formation of In Vitro–Fertilized Porcine Embryos

This study investigated the effects of spermidine (Spd) treatment on early embryonic development and blastocyst formation of porcine oocytes matured in vitro and embryos produced by in vitro fertilization (IVF). Porcine cumulus-oocyte complexes were matured in vitro, fertilized, and then cultured in medium supplemented with various concentrations of Spd (0, 25, 50, 100, and 200 µM) during development to the blastocyst stage. To evaluate the influence on oocyte maturation, cumulus cell expansion and nuclear maturation rates were first examined, and no significant differences were observed between the 50 or 100 µM Spd treatment groups and the control group. However, oocytes exposed to 50 µM Spd during in vitro maturation showed a significantly higher proportion of embryos reaching the blastocyst stage (33.4±1.2%) compared with the control group (27.2±1.6%, p < 0.05). In contrast, Spd treatment for two days after IVF had no significant effect on blastocyst development. In the post-ovulatory aging (POA) model, treatment with 50 µM Spd resulted in a higher blastocyst formation rate (12.6±0.8%) compared with the untreated aged group (6.3±1.2%), although the difference was not statistically significant (p > 0.05). Hence, these findings suggest that supplementation with approximately 50 µM Spd may have a partially positive influence on the developmental potential of porcine IVF embryos, but further studies are required to confirm this effect.