Pseudomonas sp. $\beta$-1,4-Glucosidase 유전자의 Esherichia coli와 Bacillus subtilis에의 Cloning 및 발현

Cloning and Expression of $\beta$-l,4-Glucosidase Gene from Pseudomonas sp. in Escherichia coli and Bacillus subtilis

Fro the purpose of producing glouse from cellobiose or oligo saccharide and obtaining genetic information of beta-1,4-glucosidase gene, alpha beta-1,4-glucosidase gene of Pseudomonas sp. LBC505, potent cellulase complex and xylanase producing strain, was cloned in Esherichia coli and Bacillus subtilis into pUC19 and pBD64, respectively. Recombinant plasmid pGL1 contained 1.2kb EcoRI fragment was isolated from transformants forming blue color around colony on LB agar plate containing 20 ng/ml of 5-bromo-4-chloro-3-indolyl-beta-D-glucopyranoside(X-glu) and ampicillin.