Brevibacterium ketoglutamicum을 이용한 L-Ornithine 생산 연구 PART II : L-Arginine 제한공급에 의한 :-Ornithine 유가식 발효생산

High Production of L-Ornithine by L-Citrulline Auxotroph of Breviabcterium ketoglutamicum : PART II : Production of L-Ornithine by Controlled Feeding of L-Arginine

A highly productive fed-batch fermentation process was developed for the production of L-ornithine by using a new stabilized strain, Breviabcterium ketoglutamicum BK52. Fed-batch cultures with a continuous feeding of the complex medium were conducted on various operating conditions. The optimal concentration of phosphate in the complex medium was 2.1g/L. The optimal feeding rate of L-arginine was 0.028g/L/hr. The optimal feeding point of the complex medium was determined to be at 40 OD of the cell mass. The final L-ornithine concentrations within 64hrs of cultivation in 5 and 50 liter fermenters were 73g/L and 71g/L, respectively. The maximum overall L-ornithine productivity was 1.14g/L/hr which was about 2 times higher than that of the conventional fed-batch culture with intermittent feeding. The overall productivity of the fermentation system is remarkably improved by employing the optimized conditions, and it offers a significant potential for industrial application.