재래식 메주로부터 분리한 Mucor racemosus f. racemosus PDA 103 유래 Fungal Protease의 정제

purification of Fungal Protease Produced by Mucor racemosus f. racemosus PDA 103 from Korean Traditional Meju

The protease produced by Mucor racemosus f. racemosus PDA 103 from meju was purified by precipitating with 80% saturated ammonium sulfate, CM Sephadex C-50 ion-exchange chromatography, and secondary Sephadex G-100 gel filtration chromatography. The specific activity of the purified enzyme was 60.1unit/mg protein and the purification fold of the enzyme was 83.5. The molecular weight of the enzyme was estimated 33,746Da and the enzyme was elucidated as monomer by LC-MS and SDS-PAGE. The number of amino acids was evaluated about 330 residues.

(0)

(0)

(0)

(0)