주정과 구연산 및 식이성 칼슘소재를 처리한 멸치분말이 흰쥐의 칼슘대사에 미치는 영향

Effect of Anchovy Treated with Ethanol, Citric Acid and Dietary Calcium Supplements on Calcium Metabolism in Rats

본 연구는 주정과 구연산을 처리한 대멸분말과 함께 식이성 칼슘소재가 SD계 흰쥐의 칼슘대사에 미치는 영향을 조사하기 위하여 수행되었다. 실험식이는 대델분말의 칼슘함량을 고려하여 식이중의 칼슘함량이 0.1%가 되도록 semi-purified diet(AIN-diet, 1977)에 건조대별분말을 첨가한 대조군(C), 주정-구연산처 리군(EC), 주정-구연산+CPPS처리군(ECC), 젖산칼슘첨가군(CL) 및 인산칼슘침가군(CP)으로처리하여 5주동안 실험한 결과는 다음과 같다. 증체랑은 EC군이 ECC군과 일반적인 칼슘소재로 이용되는 젖산칼슘군(CL)과 인산칼슘군(CP)에 비해 유의하게 높았고(p<0.05), 식이효율(FER)은 차이가 없었다. 생체내외(in vitro 을 in vivo) 칼슘흡수율은 CPP를 처리한 ECC군이 각각 20.4%,28.4%로 실험군중 가장 높았다(p<0.05). 혈당치는 CL군(105.7 mg/dL)이 대조군(89.5 mg/dL)보다 유의하게 높았고 (p<0.05), TC농도는 EC군(75.1 mg/dL)이 CP군(65.6 mg/dL)보다 높았으며, TG농도는 CP군(33.5 mg/dL)이 통계적으로 가장 낮았다(p<0.05). ALP활성과 057농토는 실험군간 차이가 없이 CL군이 대조군보다 다소 높았다. 혈중 Ca농토는 칼슘흡수율이 가장 낮은 대조군(C)이 10.82 mg/dL로 유의하게 낮았고, EC군과 ECC군이 가장 높았다(p<0.05). 대퇴골무게는 CP군이 가장 낮았고(p<0.05),길이는ECC군이 가장 길었다(P<0.05). 골밀도는 CP군$(0.1116\;g/cm^2)$이 가장 낮았던 반면, ECC군$(0.1149\;g/cm^2)$이 가장 높았다. 이상의 결과에서 CPPs를 첨가한 ECC군이 생체내의 칼슘흡수율과 혈중 Ca농도 및 대퇴골의 길이와 밀도 등에 유의한 상승효과를 미친 것으로 나타났다. 향추 본 연구결과는 칼슘흡수율을 높이고 색택의 개선 및 관능이 향상된 기능성제품 개발의 기초자료로 활용될 수 있을 것으로 사료된다.

This study was performed to investigate the effect of ethanol and citric acid-treated anchovy, caseino-phosphopeptides (CPPs), calcium lactate, and calcium phosphate as dietary calcium supplements on calcium metabolism in rats for 5 weeks. Experimental animals were randomly assigned to five treatments with 15 heads of SD male rats (mean body wt. of 100 g) in each group. The experimental diets were as follows; dried large anchovy powder (C) as control, ethanol+citric acid group (EC), ethanol+citric acid+cpps group (ECC), calcium lactate group (CL) and calcium phosphate group (CP), which were formulated with commercial semi-purified Chow diet, while maintaining the same level of calcium in all diets (1%) groups. The weight gain of EC group was significantly higher than ECC, CL and CP groups (p<0.05), food efficiency (FER) was not different. In vitro and in vivo calcium absorption rates of ECC group treated with citric acid and CPPs were 20.4 and 28.4%, respectively, and the highest among the experimental groups (p<0.05). The blood glucose levels of CL group (105.7 mg/dL) was significantly higher than control group (98.5 mg/dL). In terms of serum lipids, total-cholesterol concentration of EC group (75.1 mg/dL) was significantly higher than CP group (65.6 mg/dL) and triglyceride concentration of CP group (33.5 mg/dL) was the lowest (p<0.05). ALP activity and 057 level were not different among experimental groups. The serum calcium concentration of control group (C) was the lowest among groups (p<0.05). The femur weight of CP group was the lowest (p<0.05) and the femur length of ECC group is the longest (P<0.05). The bone density of CP group $(0.1116\;g/cm^2)$ was the lowest while ECC group $(0.1149\;g/cm^2)$ was the highest, and the bone density was increased by added CPPs. These data demonstrated that ECC group significantly increased in vitro and in vivo calcium absorption rate, serum Ca level, and the length and bone density of femur.