상황버섯(Phellinus linteus) 추출물의 생리활성

Biological Activities of Extracts from Phellinus linteus

상황버섯을 에탄올 농도별로 추출하여 항산화 효과와 생리활성을 측정해 보았다. 농도별로 추출한 상황버섯의 페놀 함량은 추출에탄올 농도가 짙어질수록 페놀함량이 높게 나타났으며 그 중 80%에탄올 추출물에서 28.36mg/100ml로 가장 높게 나타났다. 상황버섯 추출물의 수용성물질에 대한 항산화 효과는 DPPH radical소거활성이 전체적으로 80%가 넘는 높은 저해율을 나타났고, 80% 및 90%에탄올추출물에서는 각각 94.12, 94.14%로 가장 높은 저해율을 나타내었으며, ABTS radical cation decolorization에서는 80% 에탄올 추출물에서 96.02%로 가장 높은 저해율을 나타내었다. 지용성물질에 대한 항산화효과는 antioxidant protection factor(PF)는 80% 에탄올 추출물에서 1.69로 가장 높게 나타났으며, TBARS는 80%, 90%에탄올 추출물에서 $1.15{\times}100{\mu}M,\;1.21{\times}100{\mu}M$로 대조구인 $1.95{\times}100{\mu}M$보다 낮게 나와 산화촉진인자를 binding하는 능력이 좋은 것으로 나타났다. 고혈압에 관여하는 angiotensin converting enzyme과 gout에 관여하는 xanthine oxidase의 저해효과에서는 80% 에탄올 추출물에서 각각 95.10, 85.07%로 높은 저해율을 나타내었다. 농도별로 추출한 상황버섯을 HPLC로 분석한 결과 protocatecuic acid가 가장 많이 검출되었으며, 이외에도 cafferic acid, coumaric aicd 가 존재하는 것으로 나타났다.

The biological and antioxidative activity of Phellinus linteus extracts from gradient ethanol concentrations were examined. The phenol contents of Phellinus linteus(28.36 mg/100 ml) was higher in the 80% ethanol extracts than other extracts. Electron donation ability on DPPH of 80% and 90% ethanol extracts(94.12% and 94.14% inhibition) from Phellinus linteus were the highest. The antioxidant activity against water soluble materials of Phellinus linteus ethanol extracts showed totally high inhibition rates above 80%, especially in 80% and 90% ethanol extracts, they showed each 94.12% inhibition and 94.14% inhibition. The inhibition against ABTS [2,2azinobis(3-ethylbenzothiazoline-6-sulfonic acid)] radical decolorization of 80% ethanol extracts was the highest as 96.2%. The antioxidant protection factor (PF) against lipid soluble materials was the highest in 80% ethanol extracts as 1.69 PF, and TBARS of 80% and 90% ethanol extracts were lower as $1.15{\times}100{\mu}M$ and $1.21{\times}100{\mu}M$ than control($1.95{\times}100{\mu}M$. Angiotensin converting enzyme and xanthine oxidase inhibitory activity of 80% ethanol extracts from Phellinus linteus was higher as 95.10%, 85.07% than other extracts. The results to analized of simple phenolic compounds of Phellinus linteus ethanol extrcts with HPLC showed that they were procatecuic acid, caffeic acid and coumaric acid.