국산 각막보존액에 보존 후 각막내피세포의 기능변화

목적 : 국내에서 개발중인 각막보존액(CS002, CS003)에 보존 후 각막내피의 투과성 및 각막두께를 측정하여 각막내피세포의 기능 변화에 대해 연구하고자 하였다.<BR> 대상과 방법 : 백색가토 안구를 즉시 적출한 fresh군, 안구 적출 후 24시간 동안 4℃ 습실보존군, 1, 3, 5, 7일 동안 보존한 CS002 및 Optisol군, 7, 10, 14일 동안 보존한 CS003 및 Likorol군으로 나누고 carboxyfluorescein용액을 이용하여 각막내피세포의 투과도를 구하였다. 각막두께는 경면현미경에 부착된 각막두께측정계 (미세 초점 조절기)를 이용하여 측정하였다.<BR> 결과 : fresh군의 각막내피세포 투과도는 3.64±0.33×10-4 cm/min, 24시간 4℃ 습실 보존군은 4.79±0.28×10-4 ㎝/min이었다. 5, 7일째 CS002군은 각각 5.81±0.55×10-4 cm/min, 5.65±0.58×10-4 ㎝/min으로 24시간의 4℃ 습실 보존군과는 차이가 있었으나 (P＜0.05) 같은 기간의 Optisol군과 차이는 없었다. 7일, 10일, 14일째 CS003군은 각각 4.34±0.34×10-4 ㎝/min, 4.66±0.59×10-4 cm/min, 4.66±0.27×10-4 cm/min으로 같은 기간의 Likorol군과 차이는 없었다. 각막두께는 7일째 CS002 및 Optisol군에서는 각각 417.80±19.37 ㎛ , 421.00±19.75 ㎛로 비슷한 정도의 각막두께 증가를 보였으나, 7일째 CS003군은 426.75±22.43 ㎛로 Likorol군의 476.00±40.08 ㎛ 보다 유의하게 얇게 측정되었고, 이러한 차이는 14일째까지 지속되었다 (P＜0.05).<BR> 결론 : 국산 각막보존액인 CS002, CS003의 각막내피세포 투과도에 미치는 영향은 기존의 각막보존액 Optisol, Likorol과 차이가 없었으며 국산 각막보존액에 각막을 보존한 경우 Likorol에 보존한 경우보다 각막을 보다 얇게 유지할 수 있었다.〈한안지 43(8):1362-1368, 2002〉

Purpose : To evaluate the endothelial function of cornea preserved in newly developing korean corneal storage media (CS002, CS003) by estimating the permeability of corneal endothelium and the change of corneal thickness.<BR> Methods : The cornea were divided into six experimental groups - fresh group immediately after enucleation, 4℃ moist chamber group preserved for 24 hours and 48 hours, Optisol & CS002 group for 1, 3, 5, and 7 days, and Likorol & CS003 group for 7, 10, and 14 days after enucleation, and then corneal endothelial permeability(Pac) was measured using carboxyfluorescein solution. Corneal thickness was measured using pachymeter(fine focus adjustment) of the specular microscope.<BR> Results : Corneal endothelial Pac (×10- 4 ㎝/min) was 3.64±0.33 in fresh group , 4.79±0.28 in 4℃ moist chamber group for 24 hours. Each endothelial Pac of CS002 group at 5 and 7 days was 5.81±0.55 and 5.65±0.58, which were different with 4℃ moist chamber preservation group for 24 hours(P＜0.05) but not different with Optisol groups at same days. Each endothelial Pac of CS003 group at 7, 10, and 14 days was 4.34±0.34, 4.66±0.59, and 4.66±0.27, which were not different from those of Likorol. Each corneal thickness of CS002 and Optisol group at 7days was 417.80±19.37 ㎛ and 421.00±19.75 ㎛, which were resemble increment. Corneal thickness was 426.75±22.43 ㎛ in CS003 group and 476.00±40.08 ㎛ in Likorol group at 7days. There was statistical difference between the two group(P＜0.05), and this difference was sustained for 14days (P＜0.05).<BR> Conclusion : There was no difference in the effect on corneal endothelial permeability between korean corneal storage media such as CS002 and CS003, and that of previous corneal storage media such as Optisol and Likorol. Corneal thickness of cornea preserved in korean corneal storage media was thinner than that of Likorol. J Korean Ophthalmol Soc 43(8):1362-1368, 2002