미만성 대세포림프종의 Retinoblastoma Gene 2/p130분석

8례의 diffuse large B cell lymphoma (DLBL), 2례의 Burkitt 림프종(BL)으로 진단된 동결조직에서 DNA를 추출한 다음 pRb/p130 exon 19, 20, 21, 22에 대한 PCR을 시행하여 증폭된 DNA를 이용하여 염기서열을 직접 분석한 결과 DLBL 8례와 BL 2례 모두에서 pRb2/p130 유전자의 변이가 없었다.<BR> 이러한 결과로 DLBL의 암발생에서 pRb2/p 130의 변이와는 관련이 없을 것으로 추정된다. 풍토성 BL에서는 pRb2/p130의 많은 변이가 있었다는 사전 연구결과와 비교해 볼 때 DLBL 림프종이 분자생물학적으로 풍토성 BL과는 다른 특징적인 소견으로 생각된다.

A Retinoblastoma-related gene 2/p130 (pRb2/p130) , which belongs to Rb family, was recently discovered. There were no studies about pRb2/p130 and mutation of tumor in contrast to this fact that pRb/p105 was related to various kinds of other cancer. There are many well known cancer genes. which are related to the diffuse large cell lymphoma (DLCL), but no researches have so far been made at the study of the mutation of pRb2/p130. Therefore. we have been made this study to show the features of mutation of pRb2/p130 in DLCL.<BR> For the research materials. we used the 10 frozen tissues, which were diagnosed as DLCL. Eight out of them were diffuse large B cell lymphoma (DLBL), and two were Burkitt`s lymphoma (BL). After we extracted the DNA from the frozen tissues, then we did PCR about the pRB/p130 exon 19, 20, 21. 22, after that. we directly analyzed the sequence of an amplified DNA. However, there were no mutation of pRb2/p130 in both DLBL 8 and BL 2. In this research, we can infer that there is no relationship between the cancer development of this tumor and the mutation of pRb2/p130 because there was no sign of mutation in the DLBL and sporadic BL and the exon 19, 20, 21. 22 of pRb2/p130. When we compare this with the results of a former research, which had suggested the existence of lots of mutations of pRb2/p130 in endemic (Burkitt`s lymphoma), we can find that the non-existence of mutation of pRb2/p130 at DLBL indicates that DLBL is different from the endemic and it is one of the characteristics which are different from the carcinogenesis and in the light of classification based on molecular biology.